Biochemistry Laboratory, MIT OpenCourseWare (OCW)

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Massachusetts Institute of Technology,
77 Massachusetts Avenue,
Cambridge, MA 02139-4307,

Course Description,
The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.